Active contour models for individual keratin filament tracking

Autoren Dmytro Kotsur
Rudolf E. Leube
Reinhard Windoffer
Julian Mattes
Editoren Peter M. Roth
Markus Vincze
Wilfried Kubinger
Andreas Müller
Bernhard Blaschitz
Svorad Stolc
Titel Active contour models for individual keratin filament tracking
Buchtitel Proceedings of the OAGM&ARW Joint Workshop on Vision, Automation and Robotic (OAGM&ARW 2017)
Typ in Konferenzband
Verlag Verlag der Technischen Universität Graz
ISBN 978-3-85125-524-9
Monat May
Jahr 2017
Seiten 109-110
SCCH ID# 17020
Abstract

As a major component of the cytoskeleton, keratin filaments form a branched network, which plays a significant role in the mechanical response, motion and dynamics of the cell. They undergo a complex dynamic lifecycle, which we aim to investigate by tracking individual filaments. In this paper we introduce an active contour-based tracking algorithm to analyze the motion of individual keratin filaments in sequences of confocal images. The algorithm combines parametric active contours (snakes) with Lukas-Kanade’s algorithm for optical flow calculation. We define an image preprocessing workflow to compute robustly the external energy of the snake and we impose an additional structural constraint for controlling the length of the contour.