An algorithm for individual intermediate filament tracking

Autoren Dmytro Kotsur
Roman Yakobenchuk
Rudolf E. Leube
Reinhard Windoffer
Julian Mattes
Editoren Natasha Lepore
Jorge Brieva
Eduardo Romero
Daniel Racoceanu
Leo Joskowicz
Titel An algorithm for individual intermediate filament tracking
Buchtitel Processing and Analysis of Biomedical Information - SaMBa 2018, Revised Selected Papers
Typ in Buch
Verlag Springer
Serie Lecture Notes in Computer Science
Band 11379
ISBN 978-3-030-13834-9
DOI 10.1007/978-3-030-13835-6_8
Monat March
Jahr 2019
Seiten 66-74
SCCH ID# 18058
Abstract

In this paper we propose an algorithm for automatic tracking of individual intermediate filaments (IF), which form a highly branched network inside living cells. The algorithm is based on Stretching Open Active Contours (SOAC) combined with a novel method for processing the snake’s open ends. The main element of our approach (Endpoint-Controlled Active Contours) is a newly introduced potential function, which is included into the snake’s energy term forcing its end-points to coincide with the filament’s end- or junction-points. The comparison of our method with similar tracking algorithms for the problem of IF tracking shows a significantly improved accuracy of the overall tracking.