An annotated fluorescence image dataset for training nuclear segmentation methods

Autoren Florian Kromp
Eva Bozsaky
Fikert Rifatbegovic
Lukas Fischer
Magdalena Ambros
Maria Berneder
Tamara Weiss
Daria Lazic
Wolfgang Dörr
Allan Hanbury
Klaus Beiske
Peter F. Ambros
Inge M. Ambros
Sabine Taschner-Mandl
Editoren
Titel An annotated fluorescence image dataset for training nuclear segmentation methods
Typ Artikel
Journal Scientific Data
Nummer 262
Band 7
DOI /10.1038/s41597-020-00608-w
Monat August
Jahr 2020
SCCH ID# 20056
Abstract

Fully-automated nuclear image segmentation is the prerequisite to ensure statistically significant, quantitative analyses of tissue preparations,applied in digital pathology or quantitative microscopy. The design of segmentation methods that work independently of the tissue type or preparation is complex, due to variations in nuclear morphology, staining intensity, cell density and nuclei aggregations. Machine learning-based segmentation methods can overcome these challenges, however high quality expert-annotated images are required for training. Currently, the limited number of annotated fluorescence image datasets publicly available do not cover a broad range of tissues and preparations. We present a comprehensive, annotated dataset including tightly aggregated nuclei of multiple tissues for the training of machine learning-based nuclear segmentation algorithms. The proposed dataset covers sample preparation methods frequently used in quantitative immunofluorescence microscopy. We demonstrate the heterogeneity of the dataset with respect to multiple parameters such as magnification, modality, signal-to-noise ratio and diagnosis. Based on a suggested split into training and test sets and additional single-nuclei expert annotations, machine learning-based image segmentation methods can be trained and evaluated.