Exploring sub-populations in EV samples with transient nano-particle tracking analysis (t-NTA)
Autoren |
Matthias Dorfer Thomas Hoch Clemens Helmbrecht |
Editoren |
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Titel | Exploring sub-populations in EV samples with transient nano-particle tracking analysis (t-NTA) |
Typ | sonst |
Journal | Journal of Extracellular Vesicles, Supplement 1 |
Nummer | 27783 |
Band | 4 |
Monat | April |
Jahr | 2015 |
Seiten | http://dx.doi.org/10.3402/jev.v4.27783 |
Abstract | In conventional Nanoparticle Tracking Analysis (NTA) small particles such as EVs are visualized by an ultra-microscopy set up and analyzed for size distribution from their Brownian motion (translational diffusion). Generally, sub-populations with different behavior and appearance can be distinguished by the naked eye but are difficult to quantify and to discriminate. We present the transient NTA (t-NTA) method, which is derived from the statistical learning theory t-SNE [1] and projects a multi-dimensional solution into a two-dimensional scatter plot. |