Exploring sub-populations in EV samples with transient nano-particle tracking analysis (t-NTA)
|T. Hoch, C. Helmbrecht. Exploring sub-populations in EV samples with transient nano-particle tracking analysis (t-NTA). Journal of Extracellular Vesicles, Supplement 1, volume 4, number 27783, pages http://dx.doi.org/10.3402/jev.v4.27783, 4, 2015.|
|Journal||Journal of Extracellular Vesicles, Supplement 1|
In conventional Nanoparticle Tracking Analysis (NTA) small particles such as EVs are visualized by an ultra-microscopy set up and analyzed for size distribution from their Brownian motion (translational diffusion). Generally, sub-populations with different behavior and appearance can be distinguished by the naked eye but are difficult to quantify and to discriminate. We present the transient NTA (t-NTA) method, which is derived from the statistical learning theory t-SNE  and projects a multi-dimensional solution into a two-dimensional scatter plot.