Mechanistic soft-sensor development for physiological and morphological characterization of filamentous fungi cultivations
|T. Hoch. Mechanistic soft-sensor development for physiological and morphological characterization of filamentous fungi cultivations. 2, 2014.|
Biomass properties, such as cell concentration, morphology, and cell-type often count as difficult-to-measure process parameter, yet they can provide pronounced benefits towards increasing process understanding. The objective of this project has been the development of soft-sensors for estimation of biomass concentration and the micro-morphological characteristics of P. chrysogenum during the penicillin production process. The developed soft-sensor uses a combination of measurements from various analytical devices, such as spectroscopic techniques and offgas composition, not only for process characterization by estimation of important physiological parameters, such as the specific growth rate, but also can be used for the development and implementation of real-time control strategies for increasing process efficiency.